This paper focuses on Comparison of microbial community between saliva and tooth swab samples.
Comparison of microbial community between saliva and tooth swab samples
Comparison of the salivary and dentinal microbiome of children with severe-early childhood caries to the salivary microbiome of caries-free children
Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water, which is water that has been used to rinse the oral cavity. It can be used as an alternative method for collecting saliva for oral microbiome analysis because it can be collected in a shorter time than saliva.
The purpose of this study was to verify whether mouth-rinse water is a suitable saliva substitute for analyzing the oral microbiome. We collected samples of mouth-rinsed water, stimulated saliva, unstimulated saliva, and tongue coating from 10 systemic healthy participants, and compared the microbial diversity and composition of the samples using next-generation sequencing of 16S rRNA-encoding genes.
The results showed that the microbial diversity of mouth-rinsed water was similar to that of unstimulated and stimulated saliva. Significantly higher than that of tongue-coating samples. The microbial composition at the species level of mouth-rinsed water also showed a very high correlation with the composition of unstimulated and stimulated saliva. These results suggest that the mouth-rinsed water is a suitable collection method instead of saliva for oral microbiome analysis.
Following the development of genome analysis technology using next-generation sequencing, the relationships between human microbiota and disease are being clarified. Even in the oral cavity, it has become clear that an imbalanced microbiota can contribute to various oral diseases. Such as periodontal disease, dental caries, and halitosis.